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SOLVED: Student performed a Bradford assay and determined the concentration of the protein extract was 1.0 mg/mL. The SDS-PAGE protocol first asks you to mix 25 microliters of your 1.0 mg/mL extract with 75 microliters of buffer. What volume of this protein buffer mixture will you add to your SDS-PAGE gel so that you end up with 5 micrograms of protein on your gel? Show calculations. Pretty sure it is a two-step calculation.
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