Protocol for Bradford Assay – BBS OER Lab Manual
Protocol for Bradford Assay – BBS OER Lab Manual
May 11, 2022
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part bradford assays for samplefraction protein concentration table determination of sample protein concentration via the bradford assay sample total assay assay actual dilution sample volum 58137
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SOLVED: A Bradford assay was performed to determine the protein content in some samples. A calibration curve below was prepared from the following standard solutions (table below). A least squares line was fitted to the calibration data; the equation of the line is shown on the graph:(s)Asgs VS ug Protein6S6SV14protein ug absorbance120.466 0.676 0.883 1.086 1.280.8 2 0.6Y = 0.0218x 0.4706 R² = 0.99989.36 18.72 80.82 37.440.40.2302010ug ProteinFrom the fit, the following least squares paramete
SOLVED: A Bradford assay was performed to determine the protei…
Dec 13, 2023
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