You have a recombinant plasmid containing a vector and a segment of foreign DNA, both equal sizes. Draw a picture of this recombinant plasmid labeling foreign and vector regions. Where the foreign …

Restriction maps are important when generating a construct for experimental use. Digesting the DNA sequence with the restriction enzymes will result in fragmented DNA of predictable sizes, based on …

18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme BamHI cleaves at …

You cut a plasmid with a restriction enzyme that cuts it into three pieces-one fragment is 280 base pairs, one fragment is 800 base pairs, and one fragment is 1400 base pairs.

You plan to clone exon 11 of the HEXA gene (Section C) into the multiple cloning site (MCS) of the plasmid vector pUC19 illustrated below. You PCR amplify only the 184 bp DNA region representing …

Which restriction enzyme is best suited for cloning in pUC8? Human Anatomy & Physiology (11th Edition) 11th Edition ISBN: 9780134580999

The restricition EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3' the restriction enzyme Hindll cleaves at the sequence 5'-AAGCTT-3'. A 20 kb circular plasmid containing OFP gene …

5) The restriction enzyme EcoRI recognizes the DNA sequence GAATTC and makes a staggered cut as shown in the blue figure below: GA ATTC CTTAAG If EcoRI cuts the generic fragment shown below, …

If the site where the enzyme cuts DNA (restriction site) is different, then the restriction enzyme will make the cut at each site. BamHI and HindIII are the unknown restriction enzymes in this experiment that …

Suppose that a geneticist discovers a new restriction enzyme in the bacterium Aeromonas ranidae. This restriction enzyme is the first to be isolated from this bacterial species. Using the standard …