Bio-Rad’s pGLO Bacterial Transformation and GFP Kits offer engaging and unforgettable student lab activities in which students engineer bacteria to express the Green Fluorescent Protein (GFP) and …

Lab report on E. Coli transformation using pGLO, detailing methods, results, and transformation efficiency. High school biology experiment.

The following pre-transformation observations of E. coli might provide baseline data to make reference to when attempting to determine if any genetic transformation has occurred.

Purpose: Transformation of pGLO plasmid into E. coli allows the production of our gene of interest, GFP, in E. coli. Plating the transformed cells on different media (ex. +/- arabinose) verifies the …

Selection for cells that have been transformed with pGLO DNA is accomplished by growth on ampillicin plates. Transformed cells will appear white (wild-type phenotype) on plates not containing …

pGLO Bacterial Transformation The pGLO plasmid has been designed to express green fluorescent pigment (GFP). The GFP gene was first isolated from jellyfish. With advancements in biotechnology, …

How is the pGLO plasmid introduced into the E. coli cell? The general process by which foreign DNA is introduced into a cell is called transformation.

In this lab, the pGLO plasmid has the GFP gene that codes for the green fluorescent protein and a gene that codes for a protein that gives the bacteria resistance to an antibiotic.

Explore bacterial transformation with the pGLO system. This lab manual guides students through the experiment, data analysis, and transformation efficiency calculations.