Chimeric antigen receptor (CAR)-T cell therapy has transformed the treatment landscape for hematologic malignancies. Despite its clinical success, the conventional CAR-T manufacturing process remains ...

Expression competent cells are specially engineered bacteria, typically E. coli, that are designed to optimize the expression of recombinant proteins. These cells enhance the efficiency of ...

1 Nel Hydrogen, Wallingford, CT, United States 2 Chemistry and Nanoscience Center, National Renewable Energy Laboratory (NREL), Golden, CO, United States The harmonization of testing protocols for ...

In situ T cell transfection methods overcome the complexity and high costs associated with conventional chimeric antigen receptor (CAR)-T therapy. However, the in situ CAR-T cell approach operates ...

ABSTRACT: Human recombinant epidermal growth factor (EGF) promotes cell proliferation as well as skin remodeling and healing. However, it’s unclear if EGF is stable in in vitro cell culture medium ...

This article is based on a poster originally authored by Weiping Sun, Xing Zhang, Zhaolu Yan, Chen Yu, Spencer Chiang, and Tianfu Zhang. Immunotherapy is an advanced and widely used treatment due to ...

Bone marrow plays a crucial role in maintaining hematopoietic stem cells and bone homeostasis. However, research has been hindered by a lack of effective markers and monoclonal antibodies to ...

In this protocol, we detail a seven-stage differentiation methodology for generating pancreatic delta cells (SC-delta cells) from human pluripotent stem cells (hPSCs). In the first step, definitive ...