Western blot detection methods each have their own advantages and disadvantages. Choosing the right one can be a difficult decision. The basic protocol of the western blot – also referred to as the ...

The western blot is a method for detecting the presence and size of specific proteins. Proteins are first separated according to size or other physical properties by gel electrophoresis, then ...

Western blotting is a trusted tool in clinical and research labs, but traditional workflows are hindered by limited sensitivity, labor-intensive steps and reproducibility issues. These constraints ...

Western blotting remains a cornerstone method for the detection and quantification of proteins from complex biological samples. This technique has evolved from its early qualitative roots to a more ...

The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins has been in practice for four decades. In recent ...

In this editorial, Kenneth Oh overviews the two main normalization methods used to quantify western blots and discusses common errors to avoid. What is western blot normalization? Kenneth Oh is the ...

Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample. However, in recent years the reproducibility ...

Western bolting is a well-established method that is currently relied on in biology laboratories for determining the specific proteins and their abundance within a given sample of cells or tissue.

Elucidating information about proteins present in biological samples is vital for scientists working in the field of life sciences. Whilst qualitative data can tell us which proteins are present in a ...